單色顯微鏡數碼相機DS-Qi2

弱熒光拍攝與寬視野

DS-Qi2單色相機具備高像素密度、高靈敏度與低噪聲的特性。

 

 超大規格CMOS傳感器

       繼承自專業數碼單反相機并由尼康制造的CMOS傳感器和成像技術，經過優化現已用于顯微技術。

高靈敏度  可檢測微弱熒光信號

       7.3um像素尺寸，高量子效率配合低讀出噪聲使DS-Qi2甚至可以檢測微弱的熒光信號。

的線性特性 

      可實現可靠的定量分析

       ± 1%的線性誤差，DS-Qi2是熒光強度測量的絕好工具。無論時間強度測量還是比率測量都可輕松應對。

  

 
Indian Muntjac Deer Skin Fibroblast Cells, Cytoskeletal F-actin labeled with Alexa Fluor 488

Sample courtesy of: Michael Davidson and Florida State University

 高幀率  

      即使使用熒光圖像亦可實現快速對焦

      2.2個電子讀出噪聲配合大容量像素及0.6電子暗電流使其可以拍攝極小噪聲的熒光圖像。

低噪聲 

      獲取微弱熒光的極低噪聲圖像

        2.2個電子讀出噪聲配合大容量像素，是獲取的熒光圖像具有極低的噪聲和的動態范圍。

  

   
 LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture.
A captured image is shown intensity scaled to show both the brightest and dimmest areas.
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

時間序列成像    

      使用NIS-Elements軟件進行熒光時間序列成像

       結合寬視野和高像素密度及低噪聲，DS-Qi2是時間序列拍攝應用的選擇。 

  
Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.
DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image.
Objective: CFI Plan Apochromat λ 60x oil / NA: 1.4)
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University