P003 NTA-μSphere
鎳螯合層析填料,用于純化His-Tag蛋白
affinity purification matrix for His-Tag proteins. polymer uSphere-based chromatographic matrix with High Flow Rate，High capacity and high performance.
NTA-mSphere is a nickel Affinity resin used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the mSphere ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.
IDA or NTA? Briefly, NTA chelates metal ion tighter that IDA and metal leakage low, but difficult to elute the target proteins. IDA chelates metal ion loosely and metal leakage is more but easier to elute the target proteins. Please see “NTA vs. IDA- a tale of two ligands" for details.
技術參數：
| Particle Size (mm) | Pore Size (?) | Ligａnd Density | Target Protein Binding Capacity (g/ml) | Pressure (MPa) | pH Stability | Suggested Flow Rate（cm/h） | Matrix |
| 50 | 800 | ~150mm Ni | 5~90 mg of a His-tagged protein MW 30 | 10 | 3~13 | 150-700 | Polyacrylate |
其他名稱：
Ni-NTA Agarose, Ni-NTA-Sepherose, Ni-NTA Agarose, Ni-NTA Sehperose, Chelating Sepherose, Ni-IDA Agarose, Ni-IDA -Sepherose, Ni-IDA Agarose, Ni-IDA Sehperose, Chelating Sepherose, Ni sepharose high performance, Ni-親和樹脂，Ni-親和層析凝膠，Ni-親和層析填料, IMAC-Sepherose, IMAC-Agarose,Ni Agarose, Ni-agarose, Ni-Sepherose, Ni Sepherose, Ni瓊脂糖，鎳瓊脂糖凝膠，鎳-瓊脂糖凝膠，鎳親和凝膠, IDA-Agarose, IDA Sepherose, ida nta, ida-nta, IDA-μSphere，NTA-μSphere, IDA μSphere，NTA μSphere, IDA聚合物微球，NTA聚合物微球, IDA-聚合物微球，NTA-聚合物微球, IDA-BestRose，NTA-BestRose, IDA BestRose，NTA BestRose,Ni Sepharose 6 Fast Flow, 17-5318-01, 17-5318-02, 17-5318-03, 17-5318-04, 17-5318-05,Ni SepharoseHigh Performance, 17-5268-02, 17-5268-01, 17-5268-03, 17-5268-024,
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